Newcomer’s Fluid
Formula
| Material | Amount | |
|---|---|---|
| Isopropanol | 60 | mL |
| Propionic acid | 30 | mL |
| Petroleum ether | 10 | mL |
| Acetone | 10 | mL |
| Dioxane | 10 | mL |
Description
Recommended for chromosomes and the Feulgen reaction for DNA. It is said to preserve mucopolysaccharides well.
Note that dioxane is different from dioxin. There is a Wikipedia article on dioxane and another on dioxin.
Since this mixture contains suspect carcinogens and ethers, treat with caution and use in a fume chamber.
Time
A few hours to overnight, depending on tissue thickness.
Aftertreatment
Transfer to absolute ethanol or clearant.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Culling, C.F.A.,
Handbook of Histopathological and Histochemical Techniques, Ed. 3,
Butterworths, London, UK.
