|Chromium trioxide, 1% aqueous||75||mL|
|Acetic acid, glacial||5||mL|
Make solution just before use.
This solution is used for chromosomes, particularly in botany. Fixation of average sized pieces is complete overnight, and then they must be washed very well with running tap water to remove all traces of chromium trioxide.
Davenport, H.A., (1960),
Histological and histochemical techniques,
W. B. Saunders Co., London, UK., Oxford, UK.