Mann’s fluid
Formula
Solution A
| Material | Amount | |
|---|---|---|
| Distilled water | 100 | mL |
| Osmium tetroxide | 1 | g |
Solution B
| Material | Amount | |
|---|---|---|
| Distilled water | 100 | mL |
| Sodium chloride | 0.75 | g |
| Mercuric chloride | to saturation | |
Combine equal parts of solutions A and B just before use.
The working solution is stable for a few days.
Description
This solution is used for cytological components. Kiernan notes that it is suitable for the Golgi apparatus. It should fix fairly rapidly, a few to several hours, and should be washed afterwards to remove excess osmium tetroxide.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Kiernan, J.A.,
Histological & Histochemical Methods, Ed. 3,
Butterworth Heinemann, Oxford, UK.
