Hollande’s Fixative

Water 220 mL
Cupric acetate 25 g
Picric acid, sat. aqu. 780 mL
Formalin, 100% 100 mL
Acetic acid, glacial 10 mL

Dissolve the cupric acetate into the water.
Add the picric acid and filter.
Add the rest of the ingredients and mix.

Fixation time should be determined by trial.

Copper fixatives are not often used in modern histology. They have a reputation for good preservation, as the copper present may act as a mordant for some dyes, including hematoxylin. For that reason nuclear staining may be enhanced.


Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
    Hollande, (1918)
    Compte rendu hebdomadaire des séances et mémoires de la société de biologie.,
      v. 81, p. 17

    Paris, France




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