Behrens’ Fixative

Water 1 L
Cupric chloride 2 g
Cupric nitrate 2 g
Phenol 10 g
Acetic acid, glacial 10 mL

Fixation time should be determined by trial.

Copper fixatives are not often used in modern histology. They have a reputation for good preservation, as the copper present may act as a mordant for some dyes, including hematoxylin. For that reason nuclear staining may be enhanced.


Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
    Mayer, (1920)
    Berlin, Germany




Translate in
Google Translate