Skip to main content

Behrens’ Fixative

Behrens’ Fixative

Formula

MaterialAmount
Water1L
Cupric chloride2g
Cupric nitrate2g
Phenol10g
Acetic acid, glacial10mL

Description

Fixation time should be determined by trial.

Copper fixatives are not often used in modern histology. They have a reputation for good preservation, as the copper present may act as a mordant for some dyes, including hematoxylin. For that reason nuclear staining may be enhanced.

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.

References

  1. Gray, Peter. (1954)
    The Microtomist’s Formulary and Guide.
    Originally published by: The Blakiston Co.
    Republished by: Robert E. Krieger Publishing Co.
    Citing:
    Mayer, (1920)
    Zoomikrotechnik
    Berlin, Germany