Aoyama’s Fluid
Formula
| Material | Amount | |
|---|---|---|
| Cadmium chloride | 10 | g |
| Strong formalin | 150 | mL |
| Distilled water | 850 | mL |
Description
The formula specifies that the strong formalin should be neutralized. Using a freshly purchased solution may avoid this, but if it does not, neutralize by layering the strong formalin over calcium carbonate (marble chips or crushed oyster shells) overnight in a loosely covered container under a fume hood.
Never do this in a closed container. Enough carbon dioxide can be generated for the pressure to break a glass jar.
This fixative is useful for the Golgi apparatus and general morphology. Average-sized pieces should be fixed within 4 to 12 hours. There is no special after-treatment.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- McManus, J.F.A. and Mowry, R.W., (1960),
Staining methods, histologic and histochemical,
Harper & Row, New York, NY, USA.
