Pectin Solution
Sometimes strange procedures are recommended.
This is a pectin solution that is claimed to be quite effective. Spread a small amount thinly on a clean, grease-free slide, pick up from a waterbath and bake on as usual. Compare with amylopectin.
Presumably, commercial pectin solutions (Certo and others in the USA) used for making jams and jellies could also be used in a similar fashion, although a preservative may need to be added to them. Remember that starch-based materials may be oxidizable to aldehydes so that any staining method which demonstrates aldehydes will give background staining.
Hollande’s Formula
| Material | Amount | |
|---|---|---|
| Garlic Cloves | 50 | g |
| Water | 80 | mL |
| Chloroform | 1 | ml |
Procedure
- Crush the garlic cloves in a pestle and morter.
- Add the water and chloroform and grind together to extract as much material as possible.
- Leave 24 hours, then filter.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Citing:–
Hollande (1911)
Archives d’anatomie microscopique. v. 13, p. 171.
Paris, France
