Degreasing is a technique used primarily in some of the more demanding trichrome methods for the demonstration of fibrin, but the general principles are applicable to many trichrome methods. The process removes lipid materials associated with tissue components which can inhibit penetration of aqueous fixatives and dyes. Secondary fixation of sections with a strongly precipitant fixative is an integral part of the technique.
Lendrum, A.C., Fraser, D.S., Slidders, W., and Henderson, R. (1962)
Studies on the character and staining of fibrin,
Journal of Clinical Pathology, vol. 15, p. 401