Carbol Fuchsin Variants
|Basic fuchsin – Mixture or 42510||New fuchsin – 42520|
|Night blue – 44085||Victoria blue R – 44040|
|Auramine O – 41000||Rhodamine B – 45170|
|Ziehl||Basic fuchsin||1 g||5 g||90%, 10 mL||100 mL|
|Albrecht||Basic fuchsin||4 g||8 g||95%, 20 mL||100 mL|
|Davalos||Basic fuchsin||10 g||5 g||Abs, 1 mL||100 mL|
|Goodpasture||Basic fuchsin||0.5 g||1 g||95%, 20 mL||80 mL||Aniline, 1 mL|
|Huntoon||Basic fuchsin||0.6 g||3.75 g||75 mL||Glycerol, 25 mL|
|Kinyoun||Basic fuchsin||4 g||8 mL||95%, 20 mL||100 mL||Molten phenol|
|Muller||Basic fuchsin||4 g||8 g||95%, 20 mL||100 mL||Tergotol 7, 0.1 mL|
|Pottenger||Basic fuchsin||1.6 g||5 g||95%, 15 mL||80 mL|
|Verhoeff||Basic fuchsin||2 g||25 mL||Abs, 50 mL||Molten phenol|
|Fite||New fuchsin||0.5 g||5 g||90 mL||Methanol, 10 mL|
|Putt||New fuchsin||1 g||5 g||90 mL||Methanol, 10 mL|
|Tilden||New fuchsin||0.5 g||5 g||90 mL||Methanol, 10 mL|
|Lillie||Night blue||1 g||5 g||Abs, 10 mL||90 mL|
|Lillie||Victoria blue R||1 g||5 g||Abs, 10 mL||90 mL|
|Kuper May||Auramine O
|10 mL||95%, 20 mL||50 mL||Glycerol, 75 mL.
Different instructions have been given for compounding. Ziehl's solution is prepared according to instruction #1. Others specify instructions similar to #2. Verhoeff's follows #3.
Phenol has a density of 1.07 grams per mL. Melting phenol and measuring volumetrically compared to simply weighing gives a difference of 7%. This is not likely to cause staining failures. As an example, Ziehl's formula requires 5 grams phenol, but if 5 mL is measured volumetrically the amount used would be 5.35 grams. In most cases this difference is likely insignificant. An exception would be Verhoeff's as 25 mL molten phenol equates to 26.75 grams.
Because of the high ethanol content an open flame should not be used with Verhoeff's solution.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide. p.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Culling, C.F.A., Alison, R.T. and Barr, W.T. (1985)
Cellular Pathology Technique, 4th ed.
Butterworths, London, UK.
Putt, F.A., (1972)
Manual of histopathological staining methods
John Wiley & Sons, New York, NY, USA
Humason, G.L., (1967)
Animal Tissue Techniques
W.H. Freeman & Co., San Francisco, CA, USA
Histopathologic Technic and Practical Histochemistry
McGraw-Hill, New York, USA