Schiff's Original Reagent
|Distilled water||100 mL|
|Sulphur dioxide gas||cylinder|
Dissolve the pararosanilin into the distilled water.
Under a fume hood, slowly bubble sulphur dioxide gas through the solution.
Stopper well and store in the dark for one to two days.
If not water clear, add activated charcoal and shake for about a minute.
Filter the solution.
Store at 0-5°C in a tightly stoppered bottle.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Schiff, (1886). Annals de chimie (et de physique), v. 140, p. 93