|Distilled water||100 mL|
|Potassium metabisulphite||2 g|
|Hydrochloric acid, 1N||20 mL|
|Activated charcoal (powder)||0.3 g|
Bring the water to boiling.
Remove from the heat and add the dye (be careful of foaming).
Cool to 60° C and filter.
Add the metabisulphite and the hydrochloric acid and mix well.
Leave overnight or longer.
Add the activated charcoal, shake well for about a minute and filter.
Store at 0-5°C in a tightly stoppered bottle.
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.