Cold Schiff Reagent
|Hydrochloric acid, 0.15N||100 mL|
|Sodium metabisulphite||1.9 g|
|Activated charcoal (powder)||0.5 g|
Dissolve the dye and metabisulphite in the hydrochloric acid.
Shake frequently, or place on a shaker for about 2 hours.
The solution should be clear to pale yellow or light brown.
Add the activated charcoal and shake for a minute or two.
Filter into a graduated cylinder.
Restore the volume to 100 mL with distilled water rinses of the residue.
Store at 0-5°C in a tightly stoppered bottle.
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.