This procedure is used when a treatment is to be applied which may remove the sections from the slide, usually a strong alkali, or when there is a strong possibility of a coarse precipitate forming which may settle on the tissue.
|Absolute ethanol||50 mL|
|Note: ether is explosive and should
be handled with care. Ensure there
are no open flames in the vicinity.
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.