This procedure is used in the PAS reaction to block hydroxyl and amino groups. It does this by forming esters with them. As a consequence some carbohydrates fail to react with the Schiff's reagent. This may be used as a means of identifying them, particularly if done in conjunction with other blocking procedures.
|Acetic anhydride||16 mL|
|Pyridine, anhydrous||24 mL|
Place dewaxed sections into the solution at the temperature specified for the time given.
|Glycogen||25°C||6 hours||Almost complete|
|Epithelial mucin||25°C||6 hours||complete|
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.