Kasten and Burton's
|Distilled water||300 mL|
|Sodium thiosulphate||6 g|
Dissolve the dye in distilled water.
Add the sodium thiosulphate.
The solution should decolorise immediately.
Optionally, the solution may be boiled for one minute.
The solution may be used immediately.
Humason, G. L., (1967)
Animal tissue techniques, Ed. 2
W. H. Freeman and Company, San Francisco, Ca, USA