Hotchkiss' Alcoholic PAS
It was initially thought that the periodic acid Schiff reaction could result in less glycogen being demonstrated than was actually present because it might dissolve in aqueous reagents. It is now known this is not a concern. Hotchkiss recommended an alcoholic method to ensure it did not take place. This method is now redundant.
|Alcoholic periodic acid|
|Sodium acetate buffer 0.2M||10||mL|
|Acid reducing rinse|
|Hydrochloric acid N/1||2||mL|
Presumably an alcoholic fixative should be required if glycogen were to dissolve in aqueous solutions. However, 5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are usually satisfactory, although glutaraldehyde should be avoided.
Culling C.F.A., (1963)
Handbook of histopathological and histochemical techniques Ed. 2
Butterworth, London, UK.