Fluorescent
Schiff Reagent

Solution

Ingredient	Amount
Acriflavine	1 g
Distilled water	200 mL
Potassium metabisulphite	2 g
Hydrochloric acid, 1N	20 mL

 

Preparation
Dissolve the dye in the water and mix well.
Add the hydrochloric acid and mix well.
Add the metabisulphite and mix well.
Stopper tightly and leave 48 hours in the dark.
Filter.

 

Notes

1. Acridine orange may also be used to make this solution.

 

Reference
Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.