Feulgen and Rossenbeck's
|Distilled water||200 mL|
|Sodium bisulphite, 10% aqueous||10 mL|
|Hydrochloric acid, 1N||20 mL|
|Activated charcoal (powder)||2 g|
Heat the distilled water to boiling in an oversized Erlenmeyer flask.
Take from the heat, and immediately add the dye. Be careful, as it foams.
Shake well until the dye is dissolved.
Cool to 50°C then add the hydrochloric acid and mix well.
Cool to 25°C, and add the sodium bisulphite. Mix well.
Stopper tightly, and store in the dark for one to two days.
If not water clear, add activated charcoal and shake for about a minute.
Filter the solution.
Store at 0-5°C in a tightly stoppered bottle.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Feulgen and Rossenbeck, (1924)
Zeitschrift für physikalische Chemie, Stöchiometrie und Verwandtschaftslehre, v. 135, p. 203