Fluorescent Nucleal Reaction
|Hydrochloric acid, 1N|
|Acid alcohol (1% hydrochloric acid in 70% ethanol)|
|Fluorescent Schiff reagent|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Many other fixatives are satisfactory. Fixatives containing strong acids should be avoided as this method depends on the acid hydrolysis of DNA, and acids in some fixatives may pre-hydrolyse the tissue (picric acid in Bouin's aqueous formal-picric-acetic mixture for example).
Using a BG 12 exciter filter, and OG 4 (yellow) and/or OG5 (orange) barrier filter.
DNA fluoresces yellow.
|Do not use
|Alcoholic fixatives||20 minutes to 2 hours||Formalin containing fixatives||35 minutes to 4 hours||Formalin vapour||2-8 hours|
Culling, C F A, Allison, R T, Barr, W T, (1985).
Cellular pathology technique., Ed. 4, p. 189.
Butterworths, London, England.
Last updated June 2013.