|Distilled water||200 mL|
|Potassium metabisulphite||1 g|
|Hydrochloric acid, 1N||20 mL|
|Activated charcoal (powder)||2 g|
Heat the distilled water to boiling in an oversized Erlenmeyer flask.
Take from the heat, and immediately add the dye.
Be careful, as it foams.
Shake well until the dye is dissolved.
Cool to 50°C then add the hydrochloric acid.
Shake well to dissolve.
Cool to 25°C, and add the metabisulphite.
Stopper well and store in the dark for one to two days.
If not water clear, add activated charcoal and shake for about a minute.
Filter the solution.
Store at 0-5°C in a tightly stoppered bottle.
Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.
Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA