for

Solutions

x
x
Solution A
Distilled water 100 mL
Solution B
Distilled water 100 mL
Solution C
Distilled water 100 mL
Solution D
Distilled water 100 mL
Solution E
Distilled water 100 mL
Solution F
Distilled water 100 mL

Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.

Method

  1. Bring sections to water via xylene and ethanol.
  2. Clear with xylene and mount with a resinous medium.

Expected results

Notes

 

Reference

 


 

 

Translate in
Google Translate
Instructions