Schiff Reagent

Solution

Ingredient Amount
Pararosanilin 1 g
Distilled water 200 mL
Potassium metabisulphite 1 g
Hydrochloric acid, 1N 25 mL
Activated charcoal (powder) 1 g

 

Preparation
Bring the water to boiling.
Remove from the heat and add the dye (be careful of foaming).
Cool to 50° C, add the hydrochloric acid and mix well.
Cool to room temperature, and add the metabisulphite.
Shake thoroughly and leave overnight or longer.
If the solution is discoloured (brown), add the activated charcoal, shake well and immediately filter.
Store at 0-5°C in a tightly stoppered bottle.

 

Notes

  1. The solution should be colourless or very pale amber. Brown solutions will often stain poorly. Usually these have been made from a batch of basic fuchsin containing too little pararosanilin.

 

Reference

 


 

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