|Distilled water||200 mL|
|Sodium hydrosulphite||1 g|
|Activated charcoal (powder)||1 g|
Bring the water to boiling.
Remove from the heat and add the dye (be careful of foaming).
Filter while hot.
Immediately add the hydrosulphite and activated charcoal.
Shake thoroughly, and filter.
Store at 0-5°C in a tightly stoppered bottle.
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.