for Reducing Substances
This technique demonstrates reducing substances in paraffin sections including melanin, lipofuscin and enterochromaffin.
Nuclear fast red
|Ferric chloride, 1% aqueous, fresh||30||mL|
|Potassium ferricyanide, 1% aqueous, fresh||4||mL|
|This solution should be used fresh.|
|Prepare immediately before use.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.
Last updated October, 2013.