Perls' Prussian Blue
Hemosiderin forms the body's iron stores. The iron is in the ferric state, and may be demonstrated by releasing it from hemosiderin with hydrochloric acid, forming ferric chloride. The iron reacts with potassium ferrocyanide to form ferric ferrocyanide. This is an insoluble, blue compound known as Prussian blue or Berlin blue. The intensity of the colour gives some indication as to amount, but it is qualitative only. Other sources of ferric iron will also be demonstrated.
|4FeCl3 + 3K4Fe(CN)6 = Fe4[Fe(CN)6]3 + 12KCl|
|Stock solution A|
|Stock solution B|
|Hydrochloric acid, conc.||2||mL|
|Stock solution A||1||volume|
|Stock solution B||1||volume|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Avoid iron containing materials and jars while fixing as these may contaminate the tissue. Acid containing fixatives may remove some of the iron deposits, but apart from that most are satisfactory.
Culling, C.F.A., Allison, R.T. and Barr, W.T.
Cellular Pathology Technique, Ed.4.
Butterworth, London, UK.
Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA
Last updated May 2005.