McClung's Jenner-Giemsa
Malaria Parasites & General Oversight

Solutions

Stock Giemsa
Stock Jenner
Working Jenner
Stock Jenner 1 volume
Distilled water 1 volume
Make the diluted solution just before using.
Working Giemsa
Stock Giemsa 1 volume
Distilled water 10 volumes
Make the diluted solution just before using.
Dehydration fluids
Acetone 95 volumes, Xylene 5 volumes (5% xylene in acetone)
Acetone 70 volumes, Xylene 30 volumes (30% xylene in acetone)
Acetone 50 volumes, Xylene 50 volumes (Equal parts xylene and acetone)
Acetone 30 volumes, Xylene 70 volumes (30% acetone in xylene)
Acetone 5 volumes, Xylene 95 volumes (5% acetone in xylene)

Tissue sample
A fixative containing mercuric chloride and ethanol is recommended, but many fixatives may be used, although staining results may vary. 3µ paraffin sections of neutral buffered formalin fixed tissue are usually suitable.

Method

  1. Bring sections to water with xylene and ethanol.
  2. Remove mercury pigment if necessary.
  3. Rinse well with distilled water.
  4. Place into working Jenner for 4 minutes.
  5. Without rinsing, place into working Giemsa 15-20 minutes.
  6. Rinse well with distilled water to differentiate.
  7. Dehydrate with the dehydratiion fluids in the order given above.
  8. Clear with xylene and mount with a synthetic resinous medium.

Expected results

Notes

  1. If the sections are too blue, dilute acetic acid in distilled water may be used to remove the excess (1% or less). Wash with distilled water afterwards to remove residual acid.

 

Reference
Humason, G.L., (1967)
Animal Tissue Techniques
W.H. Freeman & Co., San Francisco, CA, USA

 


 

 

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