McNamara's Giemsa
General Oversight stain

Solutions

Stock Giemsa
Working Giemsa
Stock Giemsa 10 mL
Acetone 10 mL
Methanol 10 mL
Distilled water 100 mL
Make the diluted solution just before using.
Colophonium solution
Colophonium 15 g
Absolute ethanol 100 mL
Rinse solution
Acetone 70 mL
Xylene 30 mL

Tissue sample
Helly or neutral buffered formalin fixed tissue are recommended. Thin (3µ) sections are desirable.

Method

  1. Bring sections to water with xylene and ethanol.
  2. Rinse well with distilled water.
  3. Place into working Giemsa for 10 to 15 minutes.
  4. Rinse with tap water water and check staining microscopically.
  5. Differentiate with colophonium solution until colour contrast is satisfactory.
    Control microscopically. If a film develops renew the colophonium solution.
  6. Rinse several times with rinse solution. Do not allow to dry.
  7. Rinse several times with xylene. Do not allow to dry.
  8. Mount with a synthetic resinous medium.

Expected results

 

Reference
Putt, F.A., (1972)
Manual of histopathological staining methods
John Wiley & Sons, New York, NY, USA

 


 

 

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