Nuclear Fast Red
for Nuclei


Solution A
Nuclear fast red 1 g
Potassium aluminum sulphate 50 g
Distilled water 500 mL
Dissolve the dye and alum into the water.
Boil for 5 minutes. Cool and filter.
Solution B
Tartrazine 1 g
Distilled water 1 L

Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.


  1. Perform the main staining method.
  2. Place into solution A for 5 to 10 minutes
  3. Rinse with water.
  4. Optionally, place into solution B for 30 seconds.
  5. Rinse with distilled water.
  6. Return to the main staining method, or
  7. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results


Kiernan. J.A., (1999)
Histological and histochemical methods: Theory and practice, Ed. 3
Butterworth Heinemann, Oxford, UK.




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