Kiernan's Eriochrome Cyanin
Substitute for alum hematoxylin in the H&E stain
|Eriochrome cyanine R||1.0||g|
|Ferric chloride, 5.6%||20||mL|
|Sulphuric acid, conc.||2.5||mL|
|Distilled water||up to 500||mL|
|Hydrochloric acid, conc.||5||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Kiernan. J.A., (1999)
Histological and histochemical methods: Theory and practice, Ed. 3
Butterworth Heinemann, Oxford, UK.
Last updated May 2005.