Kiernan's Eriochrome Cyanin
for Nuclei
Substitute for alum hematoxylin in the H&E stain
Solutions
Solution A | ||
Eriochrome cyanine R | 1.0 | g |
Ferric chloride, 5.6% | 20 | mL |
Sulphuric acid, conc. | 2.5 | mL |
Distilled water | up to 500 | mL |
Solution B | ||
Hydrochloric acid, conc. | 5 | mL |
Ethanol, abs. | 500 | mL |
Distilled water | 500 | mL |
Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Other fixatives are likely to be satisfactory.
Method
Expected results
Notes
Reference
Kiernan. J.A., (1999)
Histological and histochemical methods: Theory and practice, Ed. 3
Butterworth Heinemann, Oxford, UK.