Lendrum & Mcfarlane
Iron Alum-Celestine Blue for Nuclei
|An eosin solution, or other counterstain|
|Iron alum-celestine blue|
|Ferric ammonium sulphate||5||g|
|Celestine blue B||0.5||g|
|Dissolve the ferric ammonium sulphate in the distilled water.|
|Add the celestine blue B. Boil for 5 minutes. Cool and filter.|
|Add the glycerol.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Lendrum and McFarlane, (1940)
Journal of Pathology and Bacteriology, v. 50, pp. 381
Last updated May 2005.