Shokeir & Elgaboury's
Alloxan-Schiff for Rabies Virus

Solutions

Schiff reagent
Alloxan solution
Alloxan 1 g
Distilled water 100 mL

Tissue sample
Best results are obtained with 5 µ paraffin sections fixed in one of the following:

Method

  1. Bring sections to water via xylene and ethanol.
  2. Place into the alloxan solution at 37°C for 24 hours.
  3. Wash gently with tap water for 5 minutes.
  4. Place into Schiff's reagent for 15 minutes in the dark.
  5. Wash with running tap water for 10 minutes.
  6. Dehydrate with ethanol, clear in xylene and mount with a resinous medium (Canada balsam specified).

Expected results

Notes

  1. The authors note that this technique will demonstrate fixed rabies virus, but is not specific for them.

 

Reference
Shokeir, A. A., and Elbagoury, S. M.
Alloxan-Schiff reagent for the detection of fixed rabies virus in the nerve cell
J. med. Lab. Tech. (1966) v23, p. 191-193

 


 

 

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