This method demonstrates fungi, and may also be called a Fluorescent Gridley or CAFS.
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Using a BG 12 exciter filter, and OG 4 (yellow) and/or OG5 (orange) barrier filter.
Fungi fluoresce yellow with acriflavine and green-red with acridine orange.
No specific reference is given for this method.
A similar technique using periodic acid as the oxidant is found in:–
Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.
Last updated May 2005.