Chiffelle and Putt's Oil Red O
This technique may be used for the demonstration of fats in unfixed or fixed, cryostat or frozen sections, whether mounted on slides or free floating. It may also be used for the demonstration of lipids that resist solvent extraction during paraffin processing. Precipitation of the dye onto the glass slide and tissue is not usually seen.
|Oil red O||1||g|
|Add the dye to the propylene glycol, and heat to about 100°C. Mix well until saturated.|
|Flter while hot using a fast paper. Cool to room temperature, and refilter using a vacuum pump.|
|This solution keeps for a long time (years).|
Usually unfixed, mounted cryostat sections are used, but formalin fixed, free floating sections are stained satisfactory. Paraffin processed sections may be stained to demonstrate solvent resistant lipids, but see the notes. Sections should be 10 µ thick.
Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.
Last updated May 2005.