Smith's Vanadium Hematoxylin
for Basic Proteins including Histones
When differentiated with dilute lithium carbonate this solution demonstrates basic proteins, including nuclear histones.
|Ethanol, 100%||5 mL||Solvent|
|Distilled water||35 mL||Solvent|
|Ammonium meta-vanadate||200 mg||Solvent|
Dissolve the hematoxylin in the ethanol, then add the glycerol and water in sequence.
Add the ammonium vanadate and stir for 30 minutes.
The ammonium vanadate may not completely dissolve.
The pH is 6.2
Paraffin sections of Schaudinn, Bouin or formalin fixed tissues are suitable. Sections of Zenker or Helly fixed tissues must be soaked in saturated aqueous lithium carbonate for 4 hours to stain satisfactorily.
|Differentiation times in lithium carbonate|
|Formalin, 10%||2 minutes|
Smith, A. A., (1995)
A vanadate hematoxylin stain for basic proteins.
Biotechnic and Histochemistry, v. 70, Nº 5, p. 5.