logo

Yasvoyn's
Iron Hematoxylin

 

Solution A         Function
Hematoxylin 0.1 g Dye
Distilled water 100 mL Solvent
 
Solution B    
Ferric ammonium sulphate 2.5 g Mordant
Distilled water 100 mL Solvent

 

Compounding procedure
Make each solution seperately.

 

Use

  1. Bring sections to water via xylene and ethanol
  2. Stain in the working solution for 2 - 5 minutes
  3. Rinse with water
  4. Differentiate with70% ethanol if required
  5. Dehydrate with ethanol
  6. Clear with xylene
  7. Mount with a resinous medium

 

Notes
.

 

Reference
The Microtomist's Formulary and Guide
Peter Gray
Original (1954), The Blakiston Co., Inc.
Reprinted (1975), Robert E. Kreuger Publishing Co., Inc.
Huntington, New York.

citing:
   

 


Return to hematoxylin index.

Return to Home Page

Last updated May 2005.
 

Translate in
Google Translate
Instructions