Yasvoyn's
Iron Hematoxylin

Solution A	Amount	Function
Hematoxylin	0.1 g	Dye
Distilled water	mL	Solvent
Solution B	Amount	Function
Ferric ammonium sulphate	2.5 g	Mordant
Distilled water	100 mL	Solvent

 

Compounding procedure
Make each solution seperately.
For use, add solution B drop by drop to 20 drops of solution A until it just remains blue .
The working solution may be used immediately, but is not stable for long.

Method

1. Bring sections to water with xylene and ethanol.
2. Place into the staining solution for 2-5 minutes.
3. Rinse with water.
4. Remove excess stain with 70% ethanol if necessary.
5. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

• Nuclei  –  black
• Background  –  as counterstain or unstained

Notes

1. The stock solutions are stable for some time.
2. The working solution should be made fresh.
3. Although not specified, a tap water wash to blue the hematoxylin may be useful following step 4.
4. Following a wash to blue the nuclei, a counterstain could probably be applied, if wished, before dehydration.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Roskin, G.E, (1946). Mikroskopecheskaya technica, p. 150

 

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