Seidelin's
Iron Hematoxylin

Solution A	Amount	Function
Ferric chloride	0.6 g	Mordant
Distilled water	95 mL	Solvent
Hydrochloric acid	0.75 mL	Acidifier
Solution B	Amount	Function
Hematoxylin	1 g	Dye
100% ethanol	100 mL	Solvent

 

Compounding procedure
Make each solution seperately.
For use, combine 3 parts solution A with 2 parts solution B.
The working solution may be used immediately, but is not stable for long.

Method

1. Bring sections to water with xylene and ethanol.
2. Place into the staining solution for an appropriate time.
3. Wash well in running tap water to blue.
4. Rinse with distilled water.
5. Counterstain if desired.
6. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

• Nuclei  –  black
• Background  –  as counterstain or unstained

Notes

1. The stock solutions are stable for some time.
2. The working solution should be made fresh.
3. This is a modification of Weigert's iron hematoxylin.
4. The staining time should be determined by trial, but is likely about 10-20 minutes.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Seidelin, (1911). Parasitology, v. 4, p. 94.

 

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