Seidelin's
Iron Hematoxylin
Solution A | Amount | Function |
Ferric chloride | 0.6 g | Mordant |
Distilled water | 95 mL | Solvent |
Hydrochloric acid | 0.75 mL | Acidifier |
Solution B | Amount | Function |
Hematoxylin | 1 g | Dye |
100% ethanol | 100 mL | Solvent |
Compounding procedure
Make each solution seperately.
For use, combine 3 parts solution A with 2 parts solution B.
The working solution may be used immediately, but is not stable for long.
Method
Expected results
Notes
Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Citing:–
Seidelin, (1911).
Parasitology, v. 4, p. 94.