Rozas' iron hematoxylin is of interest as it contains aluminum and iron salts in one solution. Both are mordants used with hematoxylin, and there will presumably be some kind of competition for available binding sites which could modify the solution's staining characteristics. Since iron can be used to displace aluminum as a mordant for hematoxylin, it is likely that the solution stains more as an iron hematoxylin than as an aluminum hematoxylin.
|Ferric ammonium sulphate||1 g||Mordant|
|Aluminum chloride||1.2 g||Mordant|
|Distilled water||74 mL||Solvent|
|95% ethanol||6 mL||Solvent|
|Ferric ammonium sulphate||20 g||Mordant|
|Distilled water||100 mL||Solvent|
For solution A, dissolve the hematoxylin in the ethanol.
Dissolve the ferric ammonium sulphate and the aluminum chloride in the water.
Combine, then add the glycerol.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Zietschrift für wissenschaftliche Mikroskopie und für mikroskopische Technik,
v.52, p.1. Leipzig.