Régaud's
Iron Hematoxylin

Solution A Amount Function
Ferric ammonium sulphate 5 g Mordant
Distilled water 100 mL Solvent
 
Solution B Amount Function
Hematoxylin 1 g Dye
90% ethanol 10 mL Solvent
Distilled water 80 mL Solvent
Glycerol 10 mL Stabiliser
 
Solution C Amount Function
Potassium ferricyanide 2.5 g Bleach
Sodium borate 2 g pH control
Distilled water 100 mL Solvent

 

Compounding procedure
Make each solution seperately.
Solutions A and B may be reused provided they are not contaminated by each other.
Solution C should not be reused.

Method

  1. Bring sections to distilled water with xylene and ethanol.
  2. Place into solution A at 50°C for 30 minutes.
  3. Rinse well with distilled water.
  4. Place into solution B at 50°C for 30 minutes.
  5. Rinse well with distilled water.
  6. Place into solution C until differentiated.
  7. Wash in running tap water until blue.
  8. Counterstain as required.
  9. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

Notes

  1. The solutions are stable for some time.
  2. Solution C is Weigert's ferricyanide bleach and should be used fresh.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.

 


 

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