|Ferric ammonium sulphate||5 g||Mordant|
|100% ethanol||5 mL||Solvent|
|Distilled water||70 mL||Solvent|
Dissolve the iron alum into the water.
Dissolve the hematoxylin into the ethanol.
Combine, and leave for one week at room temperature.
Filter, and then add the other ingredients.
Combines all solutions immediately they are dissolved,
and omits the week at room temperature.
The solution is stable for a few months.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Janssen, (1897), Cellule, v.14, p.207.
Lillie & Earle, (1939), Stain Technology, v.14, p.53