Crétin's
Iron Hematoxylin

Solution A Amount Function
Ferrous sulphate 4 g Mordant
Distilled water 100 mL Solvent
 
Solution B Amount Function
Potassium ferrocyanide 2 g Mordant
Potassium ferricyanide 1 g Mordant
Distilled water 100 mL Solvent
 
Solution C Amount Function
Hematoxylin 0.5 g Dye
Distilled water 100 mL Solvent
 
Solution D Amount Function
Ferric ammonium sulphate 5 g Mordant
Distilled water 100 mL Solvent

 

Compounding procedure
Make each solution seperately.

Method

  1. Bring sections to water with xylene and ethanol.
  2. Place into solution A for 24 hours.
  3. Wash with running tap water overnight.
  4. Place into solution B for 3-6 hours.
  5. Rinse with distilled water.
  6. Place into solution C overnight.
  7. Place into solution D until differentiated.
  8. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

Notes

  1. The stock solutions should be stable for some time.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.

 


 

Translate in
Google Translate
Instructions