Iron Hematoxylin

Solution A Amount Function
Hematoxylin g Dye
100% ethanol mL Solvent
Distilled water mL Solvent
 
Solution B Amount Function
Ferric ammonium sulphate g Mordant
Distilled water 100 mL Solvent
Sulphuric acid mL Acidifier

 

Compounding procedure
Make each solution seperately.
For use, combine equal parts of solutions A and B.
The working solution may be used immediately, but is not stable for long.

Method

  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for an appropriate time.
  3. Rinse with tap water.
  4. Dip briefly in 1% hydrochloric acid in 70% ethanol.
  5. Wash well in running tap water to blue.
  6. Rinse with distilled water.
  7. Counterstain if desired.
  8. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

Notes

  1. The stock solutions are stable for some time.
  2. The working solution should be made fresh.
  3. The staining time should be determined by trial.

 

Reference

 


 

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