Surgipath SelecTech 560
Alum Hematoxylin

This formula has been reconstructed from the Surgipath web site MSDS sheets.

Ingredient Amount Function
Hematoxylin 6 g Dye
Ammonium alum 30 g Mordant
Aluminum sulphate 50 g Mordant
Distilled water 700 mL Solvent
Ethylene glycol 300 mL Solvent
Sodium iodate 1 g Oxidant
Hydrochloric acid 2 mL Acidifier

 

Compounding procedure
Dissolve the alum and aluminum sulphate in 350 mL water.
Dissolve the hematoxylin in 300 mL water.
Dissolve the sodium iodate in 50 mL water.
Combine the hematoxylin and alum solutions. Mix well.
Add the sodium iodate solution. Mix well.
Add the hydrochloric acid. Mix well.
Leave overnight.
Add the ethylene glycol. Mix well.
Filter before use.

Method

  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for the appropriate time.
  3. Rinse well with water.
  4. Differentiate with citric acid buffer.
  5. Rinse with water and blue with Trizma base solution.
  6. Rinse well with water.
  7. Counterstain with Surgipath Eosin 515 or Eosin 515 Trichrome.
  8. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

Notes

  1. This is a strong hemalum and should be used regressively.
  2. As with many strong formulations mucins may stain blue.
  3. As it contains hydrochloric acid as acidifier it may remove small calcium deposits. These are sometimes an indication of disease, in malignant breast lumps, for instance.
  4. Surgipath recommend their citric acid/citrate buffer pH 3-4 for differentiation,but acid ethanol could be used.
  5. Surgipath recommend blueing with their 1% Trizma base solution at pH 8, but other blueing agents could be used.
  6. Surgipath recommend counterstaining with their proprietary alcoholic eosin 515 solutions, but other eosin solutions could be used.

 

Reference
Surgipath web site
http://surgipath.com

 


 

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