Reddy's
Alum Hematoxylin

Ingredient Amount Function
Hematoxylin 6.4 g Dye
Ammonium alum 60 g Mordant
Ethanol, absolute 200 mL Solvent
Glycerol 160 mL Stabiliser
Distilled water 640 mL Solvent

 

Compounding procedure
Combine all the ingredients.
Mix for a day.
Leave in the dark for at least a month to ripen.

Method

  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for 3-5 minutes.
  3. Rinse with water.
  4. Differentiate lightly with 1% hydrochloric acid in 70% ethanol.
  5. Rinse with water.
  6. Blue with 0.1% sodium bicarbonate.
  7. Wash with water.
  8. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

Notes

  1. The method does not explicitely say to counterstain, although the customary dyes could be applied following step 7.
  2. The method is recommended for neurological staining.

 

Reference
Reddy, P. H., (2001)
Neurological Sciences Institute
Oregon Health and Science University
url:–  http://www.ohsu.edu/nsi/faculty/reddyh/lab/prothema.html

 


 

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