This solution is described as a modification of Mayer's hemalum, but please refer to that formula.
|Hematoxylin, aged 10% ethanolic||16 mL||Dye|
|Ammonium alum||60 g||Mordant|
|Distilled water||1 L||Solvent|
|Sodium iodate||0.25 g||Oxidant|
|Citric acid, 5% aqueous||7 mL||Acidifier|
|Chloral hydrate||50 g||Stabiliser|
Dissolve the alum in the water using low heat, but do not boil.
Add the hematoxylin solution and mix well.
Add the sodium iodate and dissolve.
Leave for 30 minutes.
Add the chloral hydrate and dissolve.
Add the citric acid and mix well.
Check that the pH is 2.45, adjust with citric acid if not.
It may be used immediately.
Pusey's modified Mayer's hematoxylin,
Journal of Histotechnology, v.2, No.2, p.54, 1979
Villaneuva, A.R., (1976)
Methods of preparing and interpreting mineralized sections of bone, in:
Proceedings of the First Workshop on Bone Morphometry
Jaworski, Z. F. G., Editor, (1976).