This formulation has been named in honor of Dr. H.K.I. McLachlan FRCPA at the request of the originator, Mike Rentsch.
|Ingredient||Var I||Var II||Function|
|Hematoxylin||2 g||2 g||Dye|
|Aluminum sulphate||17.5 g||–||Mordant|
|Ammonium alum||–||25 g||Mordant|
|Distilled water||700 mL||700 mL||Solvent|
|Glycerol||300 mL||300 mL||Stabiliser|
|Sodium iodate||0.2 g||0.2 g||Oxidant|
|Glacial acetic acid||20 mL||20 mL||Acidifier|
Dissolve the aluminum sulphate or the alum in about 500 mL water.
Add the hematoxylin and dissolve.
Add the acetic acid and mix well.
Warm the glycerol to reduce viscosity, and add to the mixture.
Mix well, then make up to 1 litre with water.
Add the iodate and mix for 5-10 minutes.
Store in a tightly capped bottle in the dark.
The solution remains usable for 2-5 years.
Australian Biostain P/L.
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