Launoy's
Alum Hematoxylin

Ingredient Amount Function
Hematein 10 g Dye
Potassium alum 5 g Mordant
Distilled water 1 L Solvent

 

Compounding procedure
Combine all ingredients, and dissolve.
The solution may be used immediately.

Method

  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for an appropriate time.
  3. Rinse well with water.
  4. Differentiate with acid ethanol if necessary.
  5. Rinse with water and blue.
  6. Rinse well with water.
  7. Counterstain if desired.
  8. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results

Notes

  1. This solution uses hematein instead of hematoxylin.
  2. Although the staining characteristics are not given, the high dye and low alum content would indicate it is likely a strong, regressive solution.
  3. The staining time should be determined by trial.
  4. Acid ethanol is 0.5% - 1% hydrochloric acid in 70% ethanol.
  5. Blueing is done with alkaline solutions such as hard tap water, Scott’s tap water substitute, 0.1% ammonia water, 1% aqueous sodium acetate, 0.5% aqueous lithium carbonate etc.

 

Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.

 


 

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