This formula is based on Langeron's 1942 (Mayer's) formula. I have referred to it as Hamilton alum hematoxylin as I received it via the internet from a histotechnologist at the Hamilton Health Sciences Corporation, Hamilton, Ontario, Canada.
|Ammonium alum||75 g||Mordant|
|Distilled water||950 mL||Solvent|
|100% ethanol||50 mL||Solvent|
|Sodium iodate||0.3 g||Oxidant|
|Glacial acetic acid||30 mL||Acidifier|
Dissolve the alum in the water.
Add the ethanol and mix well.
Add the hematoxylin and dissolve.
Add the sodium iodate and mix well.
Leave overnight in the dark to ripen.
Mix well, and add the acetic acid.
The solution may be used immediately.
Personal internet communication, source unidentified.