Grahams alum hematoxylin is a modification of Gill's alum hematoxylin, and was designed for routine staining of plant tissue. It should also be satisfactory for animal tissues. It has been formulated so that small quantities may be prepared fresh, sufficient for a single Coplin jar.
|Distilled water||750 mL||Solvent|
|Propylene glycol||210 mL||Solvent|
|Glacial acetic acid||20 mL||Acidifier|
|Aluminum sulphate||17.6 g||Mordant|
|Sodium iodate||0.2 g||Oxidant|
|Propylene glycol||100 mL||Solvent|
Combine all the ingredients and filter when dissolved.
Shake vigorously several times during 48 hours until the hematoxylin dissolves.
Solution A - 49 mL
Solution B - 1 mL
It is ready for use when an opaque dark purple.
Graham, E. T., (1991)
A quick mix aluminum hematoxylin stain
Biotechnic and Histochemistry, pp. 279-281