Not given, but based on experience:–
Dissolve the hematoxylin in ethanol.
Dissolve the alum in water.
Combine solutions, and add chloral hydrate.
- Bring sections to water with xylene and ethanol.
- Place into the staining solution for an appropriate time.
- Rinse with water and blue.
- Rinse well with water.
- Counterstain if desired.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
- Nuclei – blue
- Background – as counterstain or unstained
- The formula indicates the solution is progressive.
- As no oxidising agent is present the solution needs ageing.
- The appropriate time should be determined by trial,
but 5-10 minutes should be adequate.
- Blueing is done with alkaline solutions such as hard tap water,
Scott’s tap water substitute, 0.1% ammonia water, 1% aqueous sodium acetate,
0.5% aqueous lithium carbonate etc.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Last updated May 2005.